Journal:
Article Title: Mutations within the MGC4607 Gene Cause Cerebral Cavernous Malformations
doi:
Figure Lengend Snippet: MGC4607 expression analysis through use of RT-PCR. The expression of MGC4607 transcripts was analyzed by RT-PCR through use of the cDNA MTC1 panel (Clontech). Upper panel, MGC4607. Lower panel, β-actin. Lane 1,100-bp ladder. Lane 2, Brain. Lane 3, Heart. Lane 4, Skeletal muscle. Lane 5, Kidney. Lane 6, Liver. Lane 7, Pancreas. Lane 8, Lung. Lane 9, Placenta. In all tested cDNAs, two populations of MGC4607 transcripts existed, although at a different level, containing exon 2 (fragment from exons 1–6: 755 bp) or not containing exon 2 (fragment without exon 2: 581 bp).
Article Snippet: Human normalized multiple-tissue cDNA (MTC I [Clontech]) panel and cDNA from control EBV lymphoblasts were amplified with these two MGC4607 primer pairs and with primers specific for the β-actin gene (forward, 5′-CCAGATCATGTTTGAGACCT-3′; and reverse, 5′-ACGTCACACTTCATGATGGA-3′).
Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction